Target Name: Dengue Methyltranferase
E.C. Number: 2.1.1.57
PDB ID: 1L9K
Organism: Dengue Virus type 2 (strain Puerto Rico)
Ethiological Risk Group: n/a

Disease Information: Dengue Fever is a mosquito-born virus that is a member of the Flaviviride virus family. The virus can result in symptoms such as fever, chills, nausea, rashes, and joint pain. It is most common in areas with a high density population and is only spread by infected mosquitos. It is also one of the most common arthropod born diseases.

Dengue Overview
embopress.org

Essentiallity:
The target is essential for the synthesis of RNA and invest themselves in the replication machinery of the virus.

Essentiality

Brenda

Link
ExPasy

Structure:

external image 1l9k.pdb1-500.jpg

external image download.php?file=TMPRED.15045.6414.gif
TMPred page:
Expasy TMpred

Gene Sequence:
>fig|243365.4.peg.112|CV_0121|VBIChrVio67196_0112| SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182) [Chromobacterium violaceum ATCC 12472 | 243365.4]
atgagcaagcacatcccgcgcaagcgtttcgggcagaacttcctgcaggacgccagcgtc
atcgccggcatcgtccacgcggtcaatccgcagcctgacgacatcgtcatcgagatcggc
cccgggctgggcgccatcaccaagccgttgctggcgcgcctcaagcatctgcacgtggtg
gagatcgaccgcgacatcatcgagcggctcaaggccgaacatccggccgacaagctgacc
atccacgccggcgacgcgctggccttcgacttcgcctcggtcagcgaggcgccgctgaag
atcgtcggcaacctgccgtacaatatctccacgccgctgctgttccacctggccagctac
ggcaaccgggtgacggacatgcatttcatgctgcagaaggaagtgatagagcgcatggtg
gccgagccgtccaccgccgattacggccggctgacagtgatgctgcagtaccgtttctac
atggaaaacatcctgttcgtgccgccggaggcgttctggccgccgccgaaggtggattcc
gccgtggtgcggatgattccggcgccgggccgctgcggcaccgcgcgcgatgaggcgctg
ctggagaagctggtgagccaggcgttcgcccagcgccgcaagacgctgcgcaacaacctg
aagggcctggccgacgcggccgacctggaggcgctgggcatcgaccccggcctgcgtccg
gaaaacctgccggtggaggatttcgtccgcctggccaaccatctgcacgacaagggcgcg
cgcggctga

Protein Sequence:

>fig|243365.4.peg.112|CV_0121|VBIChrVio67196_0112| SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182) [Chromobacterium violaceum ATCC 12472 | 243365.4]

MSKHIPRKRFGQNFLQDASVIAGIVHAVNPQPDDIVIEIGPGLGAITKPLLARLKHLHVV

EIDRDIIERLKAEHPADKLTIHAGDALAFDFASVSEAPLKIVGNLPYNISTPLLFHLASY

GNRVTDMHFMLQKEVIERMVAEPSTADYGRLTVMLQYRFYMENILFVPPEAFWPPPKVDS

AVVRMIPAPGRCGTARDEALLEKLVSQAFAQRRKTLRNNLKGLADAADLEALGIDPGLRP

ENLPVEDFVRLANHLHDKGARG



Do Not Need this info for Spring (but still copy these lines to your Target page for now)
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):(link to DNA Works output text file -
that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.